Peripheral blood mononuclear cells (PBMC) are isolated by Ficoll–Hypaque. Regulatory T–cells (Tregs) are identified with fluorochrome conjugated monoclonal antibodies (mAbs) by flow cytometry analysis. The following mAbs are used for isolation of Tregs: CD4 PerCP–Cy5.5, CD25 FITC and CD127 PE. Tregs are identified as CD4+CD25+(high) CD127–/low cells and as percentage (%) of the total CD4+ cell population.
SPECIMEN TYPE: 1 x PERIPHERAL BLOOD/ EDTA (Lavender Vacutainer ) (2ml total)
SAMPLE COLLECTION: For collection of blood samples, you should arrange for a healthcare professional to draw the bloods for you according to the instructions, in a licensed medical facility.
SAMPLE STORAGE & SHIPPING: Samples should be stored and shipped at ambient temperature and away from light. Next day delivery (24-36 hours) is required. Delivery of samples to Locus Medicus should be planned between Monday and Thursday but not Friday or during the Weekend. Sample collection and pick-up by courier should take place on the same day.
RESULT TURNAROUND TIME: Between 3 and 5 working days
|Regulatory T-Cell Panel (Tregs)||RIM1004||60,00 €|